Front Cover: Preparative high‐performance liquid chromatography: Isolation of natural chemical compounds for identification and characterization

Using Isocratic Preparative Liquid Chromatography as a Quick First-Step Purification for Natural Product Isolation

IN T RO DU C T IO N Preparative chromatography is the technique of choice for isolation and purification of different types of compounds, including pharmaceutical drug candidates, target compounds from synthetic mixtures, and bioactive compounds from natural products. As a result, there has been continuous research interest in improving the fundamental knowledge and instrumentation of preparati...

Isolation and purification of in vivo-generated 25-hydroxyvitamin D2 by preparative high-performance liquid chromatography.

In order to obtain a standard compound of 25-hydroxyvitamin D2 (25-OH-D2), a method for isolating in vivo-generated 25-OH-D2 from the blood of rats or rabbits was established by using several steps of preparative high-performance liquid chromatography (HPLC). When the unsaponifiable matter of the plasma obtained from rats or rabbits receiving a large dose of vitamin D2 was applied to the prepar...

Isolation of Gregatin A from Phialophora gregata by Preparative High-Pressure Liquid Chromatography.

A method was developed for the production and purification of gregatin A from Phialophora gregata NRRL 13198 cultured on rice at 20 degrees C for 28 days. Liquid extraction followed by high-pressure liquid chromatography afforded 247.0 mg of crystalline gregatin A per kg of rice.

Isolation and Characterization of Novel Natural Compounds from Myxobacteria

extraction and characterization of allium irancum plant extract and its application in the green synthesis of silver nano particles and oxidation of thiocarbony1 compounds

سنتز سبز نانوذرات فلزی (nps) درسالهای اخیر توجه بسیارزیادی را به خود جلب کرده است. زیرا این پروتوکل کم هزینه وسازگار با محیط زیست از روش های استاندارد سنتز. در این پایان نامه ما گزارش میکنیم یک روش ساده و سازگار با محیط زیست برای سنتز نانوذرات نقره با استفاده از محلول آبی عصاره گیاه allium iranicum به عنوان یک عامل کاهش دهنده ی طبیعی. نانو ذرات نقره مشخص شد با استفاده از تکنیک های uv-visible، x...